Transcriptome analyses of mammalian tissues

Experiment ID:DRP000895
Technology:RNA-Seq
Description:In mammals, more than 60% of the genome is use for transcription for mRNAs and noncoding RNAs (ncRNAs). At present, little is known about the concerted expression of mRNAs and antisense transcripts located in their 5'-flanking regions, and the comprehensive transcriptome analysis focusing on the bidirectional transcription of mRNA and pancRNA has not been performed. We do not yet know the sequence characteristics of the bidirectionally transcribed regions. Here, we perform directional RNA sequencing in order to examine whether there is a correlation between the expression of sense and antisense RNAs at the genome wide level.
Source:DRP000895 SRA

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entries

Library ID
Identifier of the RNA-Seq library
Anat. entity ID
ID of the anatomical localization of the sample
Anat. entity name
Name of the anatomical localization of the sample
Anat. entity author annotation
Free text annotation of anatomical localization of the sample as provided by authors
Stage ID
ID of the developmental and life stage of the sample
Stage name
Name of the developmental and life stage of the sample
Stage author annotation
Free text annotation of the developmental and life stage of the sample as provided by authors
Sex
Annotation of the sex of the sample
Strain
Annotation of the strain of the sample
Time
Free text annotation of the time of sampling as provided by authors
Time unit
Unit for the time of sampling as provided by authors
Species
Physiological status
Physiological status of the organism at time of sampling
Technology
Sequencing platform
Sequenced transcript part
Possible values are: full length, all parts of the transcript are sequenced; 3': only the 3' end of the transcript is sequenced; 5': only the 5' end of the transcript is sequenced.
Fragmentation
Size of the RNA fragmentation
Run sequencing type
Paired-end or single-read run
Total read count
Total number of reads for the library.
Mapped read count
Number of reads that could be mapped to the transcriptome.
Total UMI count
Total number of individual RNA molecules (UMI) for the annotated sample. Only applicable for libraries producing UMIs.
Mapped UMI count
Number of UMIs that could be mapped to the transcriptome. Only applicable for libraries producing UMIs.
Distinct rank count
When performing a fractional ranking of the genes in the annotated sample, based on their expression level, number of distinct ranks observed, to have a value of the power for distinguishing expression levels. Used as a weight to compute a weighted mean rank accross samples for each gene and compute expression scores in Bgee.
Max rank
When performing a fractional ranking of the genes in the annotated sample, based on their expression level, maximum rank attained in the sample. Used to normalize ranks accross samples and compute expression scores in Bgee.
Link to processed expression values
See the processed expression value results for this assay
DRX002701UBERON:0000956 cerebral cortexcerebral cortexMmulDv:0000018 1-year-old stage (macaque)1 year-oldmaleNANANAMacaca mulattaNAIlluminaIllumina HiSeq 2000FULL_LENGTH100single7607428449901893002103022495Browse results
DRX002702UBERON:0000956 cerebral cortexcerebral cortexMmulDv:0000018 1-year-old stage (macaque)1 year-oldmaleNANANAMacaca mulattaNAIlluminaIllumina HiSeq 2000FULL_LENGTH50single272056320163058660002141422586.5Browse results
DRX002703UBERON:0002037 cerebellumcerebellumMmulDv:0000018 1-year-old stage (macaque)1 year-oldmaleNANANAMacaca mulattaNAIlluminaIllumina HiSeq 2000FULL_LENGTH50single232575935137825094002117122390.5Browse results
DRX002704UBERON:0002037 cerebellumcerebellumMmulDv:0000018 1-year-old stage (macaque)1 year-oldmaleNANANAMacaca mulattaNAIlluminaIllumina HiSeq 2000FULL_LENGTH50single241173391143294450002110422332Browse results
DRX002705UBERON:0000956 cerebral cortexcerebral cortexUBERON:0000104 life cycleNAmaleNANANASus scrofaNAIlluminaIllumina HiSeq 2000FULL_LENGTH100single7922090855637925002072622603.5Browse results
DRX002706UBERON:0000948 heartheartUBERON:0000104 life cycleNAmaleNANANASus scrofaNAIlluminaIllumina HiSeq 2000FULL_LENGTH50single226851010167301900001961422034Browse results