Modular transcriptional programs separately define axon and dendrite connectivity

Experiment ID:SRP184201
Technology:scRNA-Seq
Description:Patterns of synaptic connectivity are remarkably precise and complex. Single-cell RNA sequencing has revealed a vast transcriptional diversity of neurons. Nevertheless, a clear logic underlying the transcriptional control of neuronal connectivity has yet to emerge. Here, we focused on Drosophila T4/T5 neurons, a class of closely related neuronal subtypes with different wiring patterns. Eight subtypes of T4/T5 neurons are defined by combinations of two patterns of dendritic inputs and four patterns of axonal outputs. Single-cell profiling during development revealed distinct transcriptional programs defining each dendrite and axon wiring pattern. These programs were defined by the expression of a few transcription factors and different combinations of cell surface proteins. Gain and loss of function studies provide evidence for independent control of different wiring features. We propose that modular transcriptional programs for distinct wiring features are assembled in different combinations to generate diverse patterns of neuronal connectivity. Overall design: Single-cell profiling of FACS purified populations of Drosophila melanogaster T4/T5 neurons during pupal development (24h and 48h APF).
DOI:10.7554/eLife.50822
Source:SRP184201 SRA
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scRNA-Seq droplet-based processed expression values per cell population in Drosophila melanogasterscRNA-Seq droplet-based H5AD data per cell in Drosophila melanogaster

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Library ID
Identifier of the RNA-Seq library
Cell type ID
ID of the cell type of the sample
Cell type name
Name of the cell type of the sample
Cell type author annotation
Free text annotation of cell type as provided by authors
Anat. entity ID
ID of the anatomical localization of the sample
Anat. entity name
Name of the anatomical localization of the sample
Anat. entity author annotation
Free text annotation of anatomical localization of the sample as provided by authors
Stage ID
ID of the developmental and life stage of the sample
Stage name
Name of the developmental and life stage of the sample
Stage author annotation
Free text annotation of the developmental and life stage of the sample as provided by authors
Sex
Annotation of the sex of the sample
Strain
Annotation of the strain of the sample
Time
Free text annotation of the time of sampling as provided by authors
Time unit
Unit for the time of sampling as provided by authors
Species
Physiological status
Physiological status of the organism at time of sampling
Technology
Sequencing platform
Sequenced transcript part
Possible values are: full length, all parts of the transcript are sequenced; 3': only the 3' end of the transcript is sequenced; 5': only the 5' end of the transcript is sequenced.
Fractionation
Possible values are: cell, transcripts are extracted from the cell; nuclei, transcripts are extracted from the nucleus.
Fragmentation
Size of the RNA fragmentation
Run sequencing type
Paired-end or single-read run
Total read count
Total number of reads for the library.
Mapped read count
Number of reads that could be mapped to the transcriptome.
Total UMI count
Total number of individual RNA molecules (UMI) for the annotated sample. Only applicable for libraries producing UMIs.
Mapped UMI count
Number of UMIs that could be mapped to the transcriptome. Only applicable for libraries producing UMIs.
Distinct rank count
When performing a fractional ranking of the genes in the annotated sample, based on their expression level, number of distinct ranks observed, to have a value of the power for distinguishing expression levels. Used as a weight to compute a weighted mean rank accross samples for each gene and compute expression scores in Bgee.
Max rank
When performing a fractional ranking of the genes in the annotated sample, based on their expression level, maximum rank attained in the sample. Used to normalize ranks accross samples and compute expression scores in Bgee.
Link to processed expression values
See the processed expression value results for this assay
SRX5341906FBbt:00003732 T neuron T4a (Drosophila)T4aFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35666767231648704000127211251.5Browse results
SRX5341906FBbt:00003733 T neuron T4b (Drosophila)T4bFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35666767231648704000113411131.5Browse results
SRX5341906FBbt:00003734 T neuron T4c (Drosophila)T4cFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired3566676723164870400094910998.5Browse results
SRX5341906FBbt:00003735 T neuron T4d (Drosophila)T4dFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35666767231648704000110011102Browse results
SRX5341906FBbt:00003737 T neuron T5a (Drosophila)T5aFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35666767231648704000105511118Browse results
SRX5341906FBbt:00003738 T neuron T5b (Drosophila)T5bFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35666767231648704000104711083Browse results
SRX5341906FBbt:00003739 T neuron T5c (Drosophila)T5cFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired3566676723164870400093610986.5Browse results
SRX5341906FBbt:00003740 T neuron T5d (Drosophila)T5dFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired3566676723164870400094311039.5Browse results
SRX5341906FBbt:00003726 T neuron (Drosophila)filteredFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005353 pupal stage P6 (fruit fly)24h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35666767231648704000138411829Browse results
SRX5341907FBbt:00003740 T neuron T5d (Drosophila)T5dFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800122911075Browse results
SRX5341907FBbt:00003739 T neuron T5c (Drosophila)T5cFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800118211071.5Browse results
SRX5341907FBbt:00003738 T neuron T5b (Drosophila)T5bFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800125811110.5Browse results
SRX5341907FBbt:00003737 T neuron T5a (Drosophila)T5aFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800122511091.5Browse results
SRX5341907FBbt:00003735 T neuron T4d (Drosophila)T4dFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800125311268Browse results
SRX5341907FBbt:00003734 T neuron T4c (Drosophila)T4cFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800119211092.5Browse results
SRX5341907FBbt:00003733 T neuron T4b (Drosophila)T4bFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800138911215.5Browse results
SRX5341907FBbt:00003732 T neuron T4a (Drosophila)T4aFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800132511202.5Browse results
SRX5341907FBbt:00003726 T neuron (Drosophila)filteredFBbt:00001930 embryonic/larval optic lobe (Drosophila)Optic LobeFBdv:00005355 pharate adult stage P8 (fruit fly)48h APFfemaleNANANADrosophila melanogasterNA10X Genomics V2Illumina HiSeq 4000THREE_PRIMECELL0paired35867506531254782800132011603Browse results

Showing 1 to 18 of 18 entries

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